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Description of ASTM-E1286 2006ASTM E1286 - 00(2006)Standard Guide for Identification of Herpes Simplex Virus or Its DNAActive Standard ASTM E1286 | Developed by Subcommittee: E55.04 Book of Standards Volume: 14.02
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ASTM E1286Significance and Use This guide is intended for use in a biotechnology laboratory whenever the necessity arises for identifying a biological preparation believed to contain primarily HSV or its DNA. The characterization criteria used for the identification shall be performed by an individual trained in molecular virology. This guide is not meant to be used in a clinical laboratory for the identification of HSV isolated from patient specimens. 1. Scope 1.1 This guide covers laboratory characterization procedures sufficient to identify purified specimens of HSV types 1 and 2 (HSV-1 and HSV-2) or HSV-1 DNA and HSV-2 DNA used in biotechnology. For cases in which identification of HSV DNA specimens is required, the characterization criteria of and of this guide are sufficient. 1.2 This guide does not cover the identification of HSV in HSV-infected host cells. To apply this guide to such a case, it would first be necessary to isolate the virus from such samples using standard techniques of HSV purification. This guide does not cover characterization of segments of HSV DNA or of vectors containing HSV DNA segments. 1.3 This guide does not cover the specific methodology used in the identification characterization. It does not address the question of degree of purity required for herpes virus preparations: this would vary depending on the particular biotechnology use of the virus. 1.4 Warning- Laboratory work involving herpes simplex viruses can be hazardous to personnel. Biosafety 2 level facilities are recommended () . Safety guidelines shall be adhered to according to NCCLS M29-T2 and other recommendations () . This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
ASTM Standards E1873 Guide for Detection of Nucleic Acid Sequences by the Polymerase Chain Reaction Technique NCCLS Standards M29T2 Protection of Laboratory Workers from Infectious Disease Transmitted by Blood, Body Fluids, and Tissue--Second Edition; Tentative GuidelineICS Code ICS Number Code 07.100.10 (Medical microbiology) DOI: 10.1520/E1286-00R06 ASTM International is a member of CrossRef. ASTM E1286This book also exists in the following packages...Subscription InformationMADCAD.com ASTM Standards subscriptions are annual and access is unlimited concurrency based (number of people that can access the subscription at any given time) from single office location. For pricing on multiple office location ASTM Standards Subscriptions, please contact us at info@madcad.com or +1 800.798.9296.
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