ASTM E1532-00
Historical Standard: ASTM E1532-00 Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Use of the Bisbenzamide DNA-Binding Fluorochrome
SUPERSEDED (see Active link, below)
ASTM E1532
1. Scope
1.1 This practice covers procedures used for the detection of mycoplasma contamination by DNA staining.
1.2 This practice does not cover cultivation methods for the detection of mycoplasma or other methods such as biochemical detection, hybridization methodology, histochemical stains, or immunochemical methods.
1.3 This practice does not cover methods for the identification of mycoplasma organisms.
1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
1.5 This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.
ASTM Standards
E1531 Practice for Detection of Mycoplasma Contamination of Cell Cultures by Growth on Agarose Medium
Keywords
cell culture; DNA stain; indicator cells; mycoplasma;
ICS Code
ICS Number Code 07.100.10 (Medical microbiology)
DOI: 10.1520/E1532-00
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